“Bacteroides goldsteinii sp. nov. ” Isolated from Clinical Specimens of Human Intestinal Origin

Phenotypic and phylogenetic studies were performed on an unknown gram-negative, strictly anaerobic, rod-shaped bacterium isolated from human clinical specimens. This organism was indole negative, resistant to 20 % bile, produced acetic and a lesser amount of succinic acids as the major end products of glucose metabolism, and possessed a GC content of approximately 43 mol%. Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was a member of the Cytophaga-Flavobacter-Bacteroides phylum of gram-negative bacteria and formed a close association (with an average sequence similarity of 93.6%) with the second subcluster of the Porphyromonas cluster in the Bacteroides subgroup. Phylogenetically and pheno-typically it resembled Bacteroides merdae; however, a 16S rRNA gene sequence divergence of approximately 5.5 % between the unknown bacterium and B. merdae, as well as distinguishable biochemical characteristics, demonstrate that the unknown bacterium is genotypically and phenotypically distinct and represents a previously unknown subline within the Porphyromonas phylogenetic cluster. Furthermore, a DNA-DNA reas-sociation value of 17.8 % between isolates WAL 12034T (the type strain of this novel taxon) and ATCC 43184T (B. merdae type strain) also documented the separateness of the unknown species and B. merdae. Based on the phenotypic and phylogenetic findings, a new species, “Bacteroides goldsteinii sp. nov, ” is proposed. The GC content of the DNA is 43 mol % for Bacteroides. The type strain of “B. goldsteinii ” is WAL 12034T ( CCUG 48944